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2022-06-08 11:36:32 tags:原位末端凋亡检测
Genome Sequencing Services

1) Library Construction

[Introduction]

A large number of DNA fragments can be obtained by cutting the genomic DNA of the target sample with a restriction endonuclease. These DNA fragments are connected with corresponding vectors, transformed into recipient cells, and cultured to replicate in large quantities. In this way, each cell within a clone contains a specific genomic DNA fragment on the vector, and the entire clonal population contains all gene fragments of genomic DNA, that is, the genomic library.

In genome sequencing, library sequencing is the process of building a library of qualified samples and sequencing them using the illumina sequencing platform to produce high-quality sequencing data, thus providing the raw data basis for our customers by bioinformatics analysis.

[Service Process]

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[Our Advantages]

Professional and specialistic: we have more than 10 years of experience in the medical research industry, and the projects are in the charge of professional researchers with more than 3 years experience.

Transparent service: the project feedback is timely, and the progress of the experimental project is reported in stages, so that our customers have no worries.

Hardware support: the experimental site and related equipment are open to all customers. You are welcome to visit at any time

Result guarantee: the authenticity and objectivity of all experimental data are assured.

High quality resources: we have rich academic resources, covering most biomedical majors, providing personalized counseling and assistance according to different needs

Confidentiality system: we strictly implement confidentiality procedures, fully protect customers' private information, and sign confidentiality agreements in good faith

Regular invoice: A variety of convenient modes of payment like Alipay, Wechat pay and UnionPay are supported, and all modes of payment can provide regular value-added tax invoices.

[Services]

No. 

Content

1

Construction of genomic library for genome sequencing

[Sample Requirements]

1. Cell, fresh tissue, or RNA / DNA samples

2. Cell samples or fresh tissue pieces (cut into small pieces of ~ 50mg) are directly frozen in liquid nitrogen and stored at - 80 ℃.

3. Samples transportation: cells or tissue samples or RNA/DNA samples should be placed in a 1.5ml tube, sealed with sealing film, and transported with dry ice

Note: Different sequencing methods have different sample requirements. Please deliver in correspondent requirements according to the sequencing methods.

[Service Period]

14 days

[Price]

Price negotiable according to sample types and amount.

 

2) Whole genome DNA methylation sequencing

[Introduction]

Whole genome DNA methylation sequencing is the method that uses bisulfite to treat genomic DNA to convert unmethylated Chemicalbook cytosine C into uracil U. Through the whole genome re-sequencing of the treated DNA and comparison with the reference genome, the single base resolution and high-precision methylation level analysis is realized at the genome level. WGBS can achieve single base resolution and accurately analyze the methylation status of each cytosine, so as to construct a fine genome-wide DNA methylation map.

[Service Proecess]

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[Our Advantages]

Professional and specialistic: we have more than 10 years of experience in the medical research industry, and the projects are in the charge of professional researchers with more than 3 years experience.

Transparent service: the project feedback is timely, and the progress of the experimental project is reported in stages, so that our customers have no worries.

Hardware support: the experimental site and related equipment are open to all customers. You are welcome to visit at any time

Result guarantee: the authenticity and objectivity of all experimental data are assured.

High quality resources: we have rich academic resources, covering most biomedical majors, providing personalized counseling and assistance according to different needs

Confidentiality system: we strictly implement confidentiality procedures, fully protect customers' private information, and sign confidentiality agreements in good faith

Regular invoice: A variety of convenient modes of payment like Alipay, Wechat pay and UnionPay are supported, and all modes of payment can provide regular value-added tax invoices.

[Services]

No.

Content

1

Quality evaluation of the sequencing data

2

Comparison with reference genome

3

Methylation site calling

4

Methylation distribution

5

Differential methylation analysis

6

Related gene analysis

7

Multi-samples analysis

 

[Sample Requirements]

1. Cells, tissues, DNA, etc

2. Recommended starting amount (single time): DNA 10ug, sample concentration >50ng/ul, OD260/OD280 between 1.8 and 2.0

 

[Service Time]

45 days

[Price]

Price negotiable according to sample types and amount.

 

3) CHIP-seq

[Introduction]

ChIP-seq is a technology that combines ChIP with second-generation sequencing technology. It can efficiently detect DNA segments interacting with histones, transcription factors, etc. in the whole genome. ChIP is a powerful tool to study the interaction between protein and DNA in vivo. The principle of ChIP-seq is as follows: firstly, the DNA fragments bound to the target protein are specifically enriched by chromatin immunoprecipitation (ChIP), which are then purified and go through library construction; Next, the enriched DNA fragments are sequenced by high-throughput sequencing. By accurately locating millions of sequence tags on the genome, we can obtain the DNA segment information that interacts with histones and transcription factors in the whole genome.

 

[Service Proecess]

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[Our Advantages]

Professional and specialistic: we have more than 10 years of experience in the medical research industry, and the projects are in the charge of professional researchers with more than 3 years experience.

Transparent service: the project feedback is timely, and the progress of the experimental project is reported in stages, so that our customers have no worries.

Hardware support: the experimental site and related equipment are open to all customers. You are welcome to visit at any time

Result guarantee: the authenticity and objectivity of all experimental data are assured.

High quality resources: we have rich academic resources, covering most biomedical majors, providing personalized counseling and assistance according to different needs

Confidentiality system: we strictly implement confidentiality procedures, fully protect customers' private information, and sign confidentiality agreements in good faith

Regular invoice: A variety of convenient modes of payment like Alipay, Wechat pay and UnionPay are supported, and all modes of payment can provide regular value-added tax invoices.

 

[Services]

No.

Content

1

Quality evaluation of sequencing data

2

Comparison with reference genome

3

Peak calling

4

Motif analysis

5

Annotation of peak related genes

6

Differential peak analysis

7

Functional analysis of related genes

 

[Sample Requirements]

1. Cells ≥ 2 × 107; Animal tissue ≥ 200mg; Plant tissue: leaves ≥ 1.5g, stems ≥ 2 g.

2. IP product and input: concentration 0.1ng/ μL, volume ≥ 15ul, fragment length 200-500bp; Generally, the input concentration is ≥ 50ng/ μL, and the volume is ≥ 15 μL (For other information, please contact our technical support).

3. ChIP library concentration ≥ 10nM, volume ≥ 20 μL. The fragment length is 200-500bp.

 

[Service Time]

45 days

 

[Price]

Price negotiable according to sample types and amount.

 

4) IncRNA Sequencing

[Introduction]

IncRNA sequencing: through IncRNA sequencing, we can study all IncRNAs and mRNAs transcribed by specific tissues or cells of existing reference genome species at a specific period, and conduct relevant biogenic analysis.

 

[Service Processes]

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[Our Advantages]

Professional and specialistic: we have more than 10 years of experience in the medical research industry, and the projects are in the charge of professional researchers with more than 3 years experience.

Transparent service: the project feedback is timely, and the progress of the experimental project is reported in stages, so that our customers have no worries.

Hardware support: the experimental site and related equipment are open to all customers. You are welcome to visit at any time

Result guarantee: the authenticity and objectivity of all experimental data are assured.

High quality resources: we have rich academic resources, covering most biomedical majors, providing personalized counseling and assistance according to different needs

Confidentiality system: we strictly implement confidentiality procedures, fully protect customers' private information, and sign confidentiality agreements in good faith

Regular invoice: A variety of convenient modes of payment like Alipay, Wechat pay and UnionPay are supported, and all modes of payment can provide regular value-added tax invoices.

[Services]

No.

Content


lncRNA analysis

Raw data and charts for result report are provided

1

lncRNA assembly

2

lncRNA site screening

3

lncRNA coding potential analysis

4

lncRNA descriptive statistics

5

lncRNA target gene prediction

6

lncRNA conservation analysis

7

ORF length analysis and exon number  analysis

8

Expression level analysis and differential expression analysis

 

[Sample Requirements]

1. Cells, tissues, whole blood, RNA, etc

2. Recommended starting amount of total RNA is 10ug, with a minimum of 5ug. Human/rat/mouse samples can be as low as 1ug.

3. Concentration ≥ 200 ng/ul (human / rat / mouse samples can be as low as ≥ 50ng/ul)

[Service Time]

45 days

[Price]

Price negotiable according to sample types and amount.

 

5) Transcriptome sequencing

[Introduction]

The research object of transcriptome sequencing is the sum of all RNA that can be transcribed by a specific cell in a certain functional state, mainly including mRNA and non-coding RNA. Through a new generation of high-throughput sequencing, almost all transcript sequence information of a specific tissue or organ of a species in a certain state can be obtained comprehensively and rapidly. And now it has been widely used in basic research, clinical diagnosis, and drug research and development.

[Service Process]

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[Our Advantages]

Professional and specialistic: we have more than 10 years of experience in the medical research industry, and the projects are in the charge of professional researchers with more than 3 years experience.

Transparent service: the project feedback is timely, and the progress of the experimental project is reported in stages, so that our customers have no worries.

Hardware support: the experimental site and related equipment are open to all customers. You are welcome to visit at any time

Result guarantee: the authenticity and objectivity of all experimental data are assured.

High quality resources: we have rich academic resources, covering most biomedical majors, providing personalized counseling and assistance according to different needs

Confidentiality system: we strictly implement confidentiality procedures, fully protect customers' private information, and sign confidentiality agreements in good faith

Regular invoice: A variety of convenient modes of payment like Alipay, Wechat pay and UnionPay are supported, and all modes of payment can provide regular value-added tax invoices.

[Services]

No.

Content

1

Gene level analysis

2

Comparison with reference genome

3

Novel transcripts prediction

4

Alternative splicing analysis

5

SNP analysis

6

InDel analysis

 [Sample Requirements]

1. Cells, tissues, whole blood, serum, plasma, RNA, etc

2. Recommended starting amount of RNA: 2ug, minimum 1ug, concentration ≥ 50 ng/uL

 

[Service Period]

40 days

[Price]

Price negotiable according to sample types and amount.

 

6) circRNA Sequencing

[Introduction]

Circular RNA (circRNA) is a kind of special non-coding RNA, which exists in the cytoplasm of eukaryotic cells. Studies in recent years have shown that circRNA plays an important role in regulating the growth and development and the resistance to the external environment of the organisms. Functionally, circRNA molecules act as miRNA sponges in cells for they are rich in microRNA (miRNA) binding sites. Thus, it can relieve the inhibition of miRNA on its target genes and increase the expression level of target genes, which is called the competitive endogenous RNA (ceRNA) mechanism. Hence, CircRNA is also significant in regulating diseases through the interaction of miRNA associated with diseases.

[Service Process]

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[Our Advantages]

Professional and specialistic: we have more than 10 years of experience in the medical research industry, and the projects are in the charge of professional researchers with more than 3 years experience.

Transparent service: the project feedback is timely, and the progress of the experimental project is reported in stages, so that our customers have no worries.

Hardware support: the experimental site and related equipment are open to all customers. You are welcome to visit at any time

Result guarantee: the authenticity and objectivity of all experimental data are assured.

High quality resources: we have rich academic resources, covering most biomedical majors, providing personalized counseling and assistance according to different needs

Confidentiality system: we strictly implement confidentiality procedures, fully protect customers' private information, and sign confidentiality agreements in good faith

Regular invoice: A variety of convenient modes of payment like Alipay, Wechat pay and UnionPay are supported, and all modes of payment can provide regular value-added tax invoices.

 [Services]

No.

Content

1

Reference sequence comparison

2

circRNA identification

3

circRNA-derived gene analysis

4

 circRNA expression quantification

5

Differential expression analysis

6

GO and KEGG enrichment analysis

7

miRNA binding sites prediction

 [Sample Requirements]

1. Cells, tissues, whole blood, serum, plasma, RNA, etc

2. Recommended starting amount of RNA: 10ug, minimum 5ug, concentration ≥ 200 ng /uL

 

 [Service Period]

40 days

 [Price]

Price negotiable according to sample types and amount.


7) 16S/18S/ITS Amplicon Sequencing

[Introduction]

Amplicon sequencing is the sequencing of PCR products or captured fragments of specific lengths. Sequencing of 16s, ITS, and 18s hypervariable regions amplification products from environmental samples allows the study of microbial diversity and differences in community composition in the environment by detecting sequence variation and abundance in the target region.

[Service Process]

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[Our Advantages]

Professional and specialistic: we have more than 10 years of experience in the medical research industry, and the projects are in the charge of professional researchers with more than 3 years experience.

Transparent service: the project feedback is timely, and the progress of the experimental project is reported in stages, so that our customers have no worries.

Hardware support: the experimental site and related equipment are open to all customers. You are welcome to visit at any time

Result guarantee: the authenticity and objectivity of all experimental data are assured.

High quality resources: we have rich academic resources, covering most biomedical majors, providing personalized counseling and assistance according to different needs

Confidentiality system: we strictly implement confidentiality procedures, fully protect customers' private information, and sign confidentiality agreements in good faith

Regular invoice: A variety of convenient modes of payment like Alipay, Wechat pay and UnionPay are supported, and all modes of payment can provide regular value-added tax invoices.

  

[Services]

No.

Content

1

Species annotation

2

α-diversity analysis

3

β-diversity analysis

4

multivariate statistical analysis

5

CCA analysis

6

Correlation Analysis of Environmental Factors

7

VPA analysis

8

Function prediction

9

Enterotype analysis

10

Bayesian network diagram

11

Source Tracker

  

[Sample Requirements]

1. Bacteria, DNA, etc

2. Recommended starting amount of DNA: >20ng (relatively pure DNA, no host and other impurities)

 

[Service Time]

10 days

 

[Price]

Price negotiable according to sample types and amount.

 

8) Metagenome sequencing

[Introduction]

Metagenomics studies the community composition and community (gene) function of all microorganisms contained in environment samples by directly extracting and sequencing the DNA of all microorganisms from environmental samples and using the omics research strategy.

Metagenome sequencing is a new microbial research method that applies high-throughput sequencing technology to analyze the genetic material of all micro-organisms in environmental samples, including the genome of cultured and uncultured microorganisms. It aims to study microbial diversity, population structure, evolutionary relationship, functional activity, interaction and relationship with the environment.

 

[Service Process]

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[Our Advantages]

Professional and specialistic: we have more than 10 years of experience in the medical research industry, and the projects are in the charge of professional researchers with more than 3 years experience.

Transparent service: the project feedback is timely, and the progress of the experimental project is reported in stages, so that our customers have no worries.

Hardware support: the experimental site and related equipment are open to all customers. You are welcome to visit at any time

Result guarantee: the authenticity and objectivity of all experimental data are assured.

High quality resources: we have rich academic resources, covering most biomedical majors, providing personalized counseling and assistance according to different needs

Confidentiality system: we strictly implement confidentiality procedures, fully protect customers' private information, and sign confidentiality agreements in good faith

Regular invoice: A variety of convenient modes of payment like Alipay, Wechat pay and UnionPay are supported, and all modes of payment can provide regular value-added tax invoices.

[Services]

No. 

Content

1

Sequenced data quality evaluation

2

Gene prediction

3

Species and function notes

4

CARD

5

CAGMLG analysis

6

CNV(copy number variation)

7

Phage 

 

[Samples Requirements]

1. Environmental samples, DNA, etc

2. Recommended total DNA starting amount: 5 ug, minimum 0.5ug, concentration ≥ 30 ng/ul (Qubit quantitative)

[Service Time]

26.5 days

 

[Price]

Price negotiable according to sample types and amount.