clicks:99
2022-06-09 14:15:32
Genome Sequencing Services

1) Library Construction

[Introduction]

A large number of DNA fragments can be obtained by cutting the genomic DNA of the target sample with restriction endonuclease. These DNA fragments are connected with corresponding vectors, transformed into recipient cells, and cultured to replicate in large quantities. In this way, the vector of each cell in a clone contains specific genomic DNA fragments, and the whole clone population contains all gene fragments of genomic DNA, that is, genomic library.

In genome sequencing, library building sequencing is to build a library of qualified samples and sequence them using illumina sequencing platform to produce high-quality sequencing data, so as to provide raw data basis for bioinformatics analysis for customers.

 

[Service Process]


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[Our Advantages]

Professional and specialistic: we have more than 10 years of experience in the medical research industry, and the projects are in the charge of professional researchers with more than 3 years experience.

Transparent service: the project feedback is timely, and the progress of the experimental project is reported in stages, so that our customers have no worries.

Hardware support: the experimental site and related equipment are open to all customers. You are welcome to visit at any time

Result guarantee: the authenticity and objectivity of all experimental data are assured.

High quality resources: we have rich academic resources, covering most biomedical majors, providing personalized counseling and assistance according to different needs

Confidentiality system: we strictly implement confidentiality procedures, fully protect customers' private information, and sign confidentiality agreements in good faith

Regular invoice: A variety of convenient modes of payment like Alipay, Wechat pay and UnionPay are supported, and all modes of payment can provide regular value-added tax invoices.

  

[Speicific Services]

Sequence

Content

1

Construction of genomic library for genome sequencing

 

[Sample Requirements]

1. Cell, fresh tissue or total RNA / DNA samples

2. Cell samples or fresh tissue pieces (cut into small pieces of ~ 50mg) are directly frozen in liquid nitrogen and stored at - 80 ℃.

3. Sample transportation: cells or tissue samples; or total RNA/DNA samples should be placed in 1.5 ml tube, sealed with sealing film and transported with dry ice

Note: different sequencing methods have different requirements for samples. Please determine the sample delivery requirements according to the sequencing methods.

 

[Service Time]

14 days

 

[Price]

Face to face pricing according to sample types and amount.



2) Whole genome DNA methylation sequencing

[Introduction]

Whole genome DNA methylation sequencing uses bisulfite to treat genomic DNA to convert unmethylated Chemicalbook cytosine C into uracil U. Through the whole genome re-sequencing of the treated DNA and comparison with the reference genome, the single base resolution and high-precision methylation level analysis is realized at the genome level. WGBS can achieve single base resolution and accurately analyze the methylation status of each cytosine, so as to construct a fine genome-wide DNA methylation map.

[Service Proecess]

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[Our Advantages]

Professional and specialistic: we have more than 10 years of experience in the medical research industry, and the projects are in the charge of professional researchers with more than 3 years experience.

Transparent service: the project feedback is timely, and the progress of the experimental project is reported in stages, so that our customers have no worries.

Hardware support: the experimental site and related equipment are open to all customers. You are welcome to visit at any time

Result guarantee: the authenticity and objectivity of all experimental data are assured.

High quality resources: we have rich academic resources, covering most biomedical majors, providing personalized counseling and assistance according to different needs

Confidentiality system: we strictly implement confidentiality procedures, fully protect customers' private information, and sign confidentiality agreements in good faith

Regular invoice: A variety of convenient modes of payment like Alipay, Wechat pay and UnionPay are supported, and all modes of payment can provide regular value-added tax invoices.

 

[Speicific Services]

Sequence

Content

1

Quality evaluation of the sequencing data

2

Comparison with reference genome

3

Methylation site calling

4

Methylation distribution

5

Differential methylation analysis

6

Related gene analysis

7

Multi-samples analysis

 

[Sample Requirements]

1. Cells, tissues, total DNA, etc

2. Recommended starting amount (single time): DNA 10ug, sample concentration >50ng/ul, OD260/OD280 between 1.8 and 2.0

 

[Service Time]

45 days

 

[Price]

Face to face pricing according to sample types and amount.



3) CHIP-seq

[Introduction]

ChIP-seq: it is a ChIP-seq technology that combines chip with the second generation sequencing technology. It can efficiently detect DNA segments interacting with histones, transcription factors, etc. in the whole genome. ChIP is a powerful tool to study the interaction between protein and DNA in vivo. The principle of ChIP-seq is as follows: firstly, the DNA fragments bound to the target protein are specifically enriched by chromatin immunocoprecipitation (ChIP), purified and library constructed; Then the enriched DNA fragments are sequenced by high-throughput sequencing. By accurately locating millions of sequence tags on the genome, we can obtain the DNA segment information that interacts with histones and transcription factors in the whole genome.

[Service Proecess]

image.png

 

[Our Advantages]

Professional and specialistic: we have more than 10 years of experience in the medical research industry, and the projects are in the charge of professional researchers with more than 3 years experience.

Transparent service: the project feedback is timely, and the progress of the experimental project is reported in stages, so that our customers have no worries.

Hardware support: the experimental site and related equipment are open to all customers. You are welcome to visit at any time

Result guarantee: the authenticity and objectivity of all experimental data are assured.

High quality resources: we have rich academic resources, covering most biomedical majors, providing personalized counseling and assistance according to different needs

Confidentiality system: we strictly implement confidentiality procedures, fully protect customers' private information, and sign confidentiality agreements in good faith

Regular invoice: A variety of convenient modes of payment like Alipay, Wechat pay and UnionPay are supported, and all modes of payment can provide regular value-added tax invoices.

[Speicific Services]

Sequence

Content

1

Quality evaluation of sequencing data

2

Comparison with reference genome

3

Peak calling

4

Motif analysis

5

Annotation of peak related genes

6

Different peak analysis

7

Functional analysis of related genes

 

[Samples Requirements]

1. Cells ≥ 2 × 107; Animal tissue ≥ 200mg; Plant tissue quantity: leaf ≥ 1.5g, stem ≥ 2 g.

2. IP product and input: concentration 0.1ng/ul, volume ≥ 15ul, fragment length 200-500bp; Generally, the input concentration is ≥ 50ng/ul, and the volume is ≥ 15ul (Please contact our technical support for other information).

3. ChIP library concentration ≥ 10nm, volume ≥ 20 μ 50. The fragment length is 200-500bp.

 

[Service Time]

45 days

 

[Price]

Face to face pricing according to sample types and amount.


5) IncRNA Sequencing

[Introduction]

IncRNA sequencing: through IncRNA sequencing, we can study all IncRNAs and mRNAs transcribed by specific tissues or cells of existing reference genome species at a specific period, and conduct relevant biogenic analysis.

 

[Service Processes]

image.png

 

[Our Advantages]

Professional and specialistic: we have more than 10 years of experience in the medical research industry, and the projects are in the charge of professional researchers with more than 3 years experience.

Transparent service: the project feedback is timely, and the progress of the experimental project is reported in stages, so that our customers have no worries.

Hardware support: the experimental site and related equipment are open to all customers. You are welcome to visit at any time

Result guarantee: the authenticity and objectivity of all experimental data are assured.

High quality resources: we have rich academic resources, covering most biomedical majors, providing personalized counseling and assistance according to different needs

Confidentiality system: we strictly implement confidentiality procedures, fully protect customers' private information, and sign confidentiality agreements in good faith

Regular invoice: A variety of convenient modes of payment like Alipay, Wechat pay and UnionPay are supported, and all modes of payment can provide regular value-added tax invoices.

 

[Speicific Services]

Sequence

Content


lncRNA analysis

Raw data and results report chart

1

lncRNA assembly

2

lncRNA site screening

3

lncRNA coding potential analysis

4

lncRNA descriptive statistics

5

lncRNA target gene prediction

6

lncRNA conservative analysis

7

ORF length analysis and exon number analysis

8

Expression level analysis and differential expression analysis

 

[Samples Requirements]

1. Cells, tissues, whole blood, total RNA, etc

2.  Recommended starting amount of total RNA is 10ug, with a minimum of 5ug. Human / rat / mouse samples can be as low as 1ug,

3. Concentration ≥ 200 ng/ul (human / rat / mouse samples can be as low as ≥ 50ng/ul)

 

[Service Time]

45 days

 

[Price]

Face to face pricing according to sample types and amount.



5) Transcriptome sequencing

[Introduction]

The research object of transcriptome sequencing is the sum of all RNA that can be transcribed by a specific cell in a certain functional state, mainly including mRNA and non-coding RNA. Through a new generation of high-throughput sequencing, almost all transcript sequence information of a specific tissue or organ of a species in a certain state can be obtained comprehensively and rapidly. It has been widely used in basic research, clinical diagnosis and drug research and development.

 

[Our Advantages]

Professional and specialistic: we have more than 10 years of experience in the medical research industry, and the projects are in the charge of professional researchers with more than 3 years experience.

Transparent service: the project feedback is timely, and the progress of the experimental project is reported in stages, so that our customers have no worries.

Hardware support: the experimental site and related equipment are open to all customers. You are welcome to visit at any time

Result guarantee: the authenticity and objectivity of all experimental data are assured.

High quality resources: we have rich academic resources, covering most biomedical majors, providing personalized counseling and assistance according to different needs

Confidentiality system: we strictly implement confidentiality procedures, fully protect customers' private information, and sign confidentiality agreements in good faith

Regular invoice: A variety of convenient modes of payment like Alipay, Wechat pay and UnionPay are supported, and all modes of payment can provide regular value-added tax invoices.

 

[Speicific Services]

Sequence

Content

1

Gene structure level analysis

2

Comparison with reference genome

3

Prediction of new transcripts

4

Variable shear analysis

5

SNPanalysis

6

InDel analysis

 

[Samples Requirements]

1. Cells, tissues, whole blood, serum, plasma, total RNA, etc

2. Recommended starting amount of total RNA: 2ug, minimum 1ug, concentration ≥ 50 ng/uL


[Service Time]

40 days


[Price]

Face to face pricing according to sample types and amount.



1) circRNA Sequencing

[Introduction]

Circular RNA (circRNA) is a kind of special non-coding RNA, which exists in the cytoplasm of eukaryotic cells. Studies in recent years have shown that circRNA plays an important role in regulating the growth and development and the resistance against the external environment of the organisms. Functionally, circRNA molecules are rich in microRNA (miRNA) binding sites and act as miRNA sponges in cells, thus relieving the inhibition of miRNA on its target genes and increasing the expression level of target genes; This mechanism is called competitive endogenous RNA (ceRNA) mechanism. CircRNA plays an important regulatory role in diseases through the interaction of miRNA associated with diseases.

 

[Service Process]

 image.png


[Our Advantages]

Professional and specialistic: we have more than 10 years of experience in the medical research industry, and the projects are in the charge of professional researchers with more than 3 years experience.

Transparent service: the project feedback is timely, and the progress of the experimental project is reported in stages, so that our customers have no worries.

Hardware support: the experimental site and related equipment are open to all customers. You are welcome to visit at any time

Result guarantee: the authenticity and objectivity of all experimental data are assured.

High quality resources: we have rich academic resources, covering most biomedical majors, providing personalized counseling and assistance according to different needs

Confidentiality system: we strictly implement confidentiality procedures, fully protect customers' private information, and sign confidentiality agreements in good faith

Regular invoice: A variety of convenient modes of payment like Alipay, Wechat pay and UnionPay are supported, and all modes of payment can provide regular value-added tax invoices.

  

[Speicific Services]

Sequence

Content

1

Comparision of reference sequence

2

circRNA identification

3

circRNA source gene analysis

4

Quantification of circRNA expression level

5

Differential expression analysis

6

GOKEGG enrichment analysis

7

miRNA binding site forecasting

 

[Samples Requirements]

1. Cells, tissues, whole blood, serum, plasma, total RNA, etc

2. Recommended starting amount of total RNA: 10ug, minimum 5ug, concentration ≥ 200 ng /uL

 

[Service Time]

40 days

 

[Price]

Face to face pricing according to sample types and amount.



7) Amplicon 16s/18s/its Sequencing

[Introduction]

Amplicon sequencing is to sequence the PCR products or captured fragments of a specific length, and sequence the amplification products of 16s, ITS and 18s hypervariable regions of environmental samples. By detecting the sequence variation and abundance of the target region, the diversity and community composition of microorganisms in the environment can be studied.


[Service Process]

 image.png

 

[Our Advantages]

Professional and specialistic: we have more than 10 years of experience in the medical research industry, and the projects are in the charge of professional researchers with more than 3 years experience.

Transparent service: the project feedback is timely, and the progress of the experimental project is reported in stages, so that our customers have no worries.

Hardware support: the experimental site and related equipment are open to all customers. You are welcome to visit at any time

Result guarantee: the authenticity and objectivity of all experimental data are assured.

High quality resources: we have rich academic resources, covering most biomedical majors, providing personalized counseling and assistance according to different needs

Confidentiality system: we strictly implement confidentiality procedures, fully protect customers' private information, and sign confidentiality agreements in good faith

Regular invoice: A variety of convenient modes of payment like Alipay, Wechat pay and UnionPay are supported, and all modes of payment can provide regular value-added tax invoices.

 

[Speicific Services]

Sequence

Content

1

Species annotation

2

α-diversity analysis

3

β-diversity analysis

4

multivariate statistical analysis

5

CCA analysis

6

Correlation Analysis of Environmental Factors

7

VPAanalysis

8

Function prediction

9

Enterotype analysis

10

Bayesian network diagram

11

Source Tracker

 

[Samples Requirements]

1. Bacteria, DNA, etc

2. Recommended starting amount of total DNA: >20ng (relatively pure DNA, no host and other impurities)

 

[Service Time]

10 days

 

[Price]

Face to face pricing according to sample types and amount.



8) Metagenome sequencing

[Introduction]

Metagenomics studies the community composition and community (gene) function of all microorganisms contained in environmental samples by directly extracting and sequencing the DNA of all microorganisms from environmental samples and using the omics research strategy.

Macro-gene sequencing is a new microbial research method that uses high-throughput sequencing technology to sequence and analyze the genetic material of all micro-organisms in environmental samples, including the genome of culturable and unculturable microorganisms. It aims to study microbial diversity, population structure, evolutionary relationship, functional activity, interaction and relationship with the environment.

 

[Service Process]

image.png

 

[Our Advantages]

Professional and specialistic: we have more than 10 years of experience in the medical research industry, and the projects are in the charge of professional researchers with more than 3 years experience.

Transparent service: the project feedback is timely, and the progress of the experimental project is reported in stages, so that our customers have no worries.

Hardware support: the experimental site and related equipment are open to all customers. You are welcome to visit at any time

Result guarantee: the authenticity and objectivity of all experimental data are assured.

High quality resources: we have rich academic resources, covering most biomedical majors, providing personalized counseling and assistance according to different needs

Confidentiality system: we strictly implement confidentiality procedures, fully protect customers' private information, and sign confidentiality agreements in good faith

Regular invoice: A variety of convenient modes of payment like Alipay, Wechat pay and UnionPay are supported, and all modes of payment can provide regular value-added tax invoices.

 

[Speicific Services]

Sequence 

Content

1

Sequencing data quality evaluation

2

Gene prediction

3

Species and function notes

4

CARD

5

CAGMLG analysis

6

CNV(copy number variation)

7

Phage 

 

[Samples Requirements]

1. Environmental samples, total DNA, etc

2. Recommended total DNA starting amount: 5 UG, minimum 0.5ug, concentration ≥ 30 ng/ul (qubit quantitative)

 

[Service Time]

26.5 days

 

[Price]

Face to face pricing according to sample types and amount.